Flow cytometry is a powerful technique that allows for the simultaneous measurement of multiple parameters at the single-cell level. Multivariate flow cytometry (often involving more than 10 parameters) enables functional analysis and the identification of rare and specific cell populations.
1- Immunology and Immunophenotyping
Subsets of T cells including naïve, memory, and regulatory T cells.
Maturation of B cells in germinal centers and plasma cells.
Innate immune profiling of NK cells, monocytes, and dendritic cell subsets.
2- Functional Analysis
Cytokine production of IFN-γ, TNF-α, and IL-17 upon stimulation.
Phospho-signaling of pSTAT and pERK in response to drugs.
Cell cycle and proliferation analysis involving Ki-67, DAPI, and EdU integration.
3- Cancer Detection and Minimal Residual Disease (MRD)
Immunophenotyping of leukemia/lymphoma (dim populations of CD19, CD34, CD45).
Tumor microenvironment analysis (Tregs, MDSCs).
4- Stem Cell and Hematopoietic Studies
HSC markers (CD34+, CD38-, CD90+).
Pluripotency markers (Oct4, Sox2, Nanog).